Figure 2.

(A) Morphological analysis of cell death in control C2C12 myotubes (left panel, a), or exposed to 500 µM H₂O₂ for 24 h (right panel, b). Scale bar, 75 μm. (B) Representative immunoblot images of specific apoptotic markers, such as Bcl-2, Bax, and caspase-3, were evaluated in C2C12 myotubes exposed to hydrogen peroxide (500 µM for 24 h) in presence or not of tadalafil (1 µM, 30 min pre-treatment, and then combined treatments for the following 24 h). (C,D) Representative immunoblot images of total and phosphorylated form of CRYAB, evaluated in C2C12 myotubes exposed to hydrogen peroxide (500 µM) for 1 h (C) or 24 h (D), pre-treated, or not, with tadalafil. Bar diagrams represent the densitometric intensities of p-CRYAB, normalized with total CRYAB. β-actin was used as loading control. * p < 0.05 vs. ctrl; ^§ p < 0.05 vs. H₂O₂.