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. 2022 Jun 13;23(12):6584. doi: 10.3390/ijms23126584

Figure 1.

Figure 1

Construction of the Nsun6-KO mouse strain. (A) The schematic design of the CRISPR/Cas-mediated Nsun6 KO, where two sgRNAs were used to remove exon 3 and 4 of the gene. Two PCR primer pairs were designed to confirm the genotype. (B) The correct genotype of the KO mouse was confirmed: the primer pairs F1-R1 and F2-R2 were used to detect the KO and WT allele, respectively. (C) The appearance of Nsun6-KO and WT mice. (D) The bodyweight of Nsun6-KO and WT mice at the age of 8 weeks (n = 5, p > 0.5).