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. 2022 Jun 13;23(12):6584. doi: 10.3390/ijms23126584

Figure 4.

Figure 4

Nsun6 is dispensable in GC B cell response but important for antigen-specific ASC formation. (A) Flow cytometric analysis of the GC B cell (B220+CD38-FAS+) in the spleen of WT and KO mice. (B) The ratio and number of GC B cells. (C) Flow cytometric analysis of DZ (B220+ CD38-FAS+CXCR4highCD83low) and LZ (B220+CD38-FAS+CXCR4lowCD83high) B cells. (D) The proportion of DZ and LZ B cells. (E) Flow cytometric analysis of NP-specific IgG1+ B cells (B220+Dump [IgG, IgM, Gr-1, CD138, CD3]-NIP+IgG1+) in the spleen. (F) The ratio and number of NP-specific IgG1+ B cells. (G) ELISPOT assay anti-NP IgG1 Ab-secreting plasma cells in the spleen (H) Cell number of anti-NP IgG1 Ab-secreting plasma cells. In B, D, F, H, each dot represents an individual mouse. All mice were analyzed on day 10 post-immunization with NP-CGG. All data were from two independent experiments. In each statistical analysis, a two-tailed unpaired Student t-test was used. *** p < 0.001; ns, not significant (p > 0.05).