TABLE 2.
PCR primers used for each template in this study
| Genea | Location | Sequence | Polarity | Size of PCR product (bp) | Size of PCR competitor product (bp) |
|---|---|---|---|---|---|
| lukPV | 2344–2367 | 5′-ATGACTCAGTAAACGTTGTAGAT-3′ | Sense | 520 | 600 |
| X72700a | 2802–2826 | 5′-TCTATCCATTTCACTTTGATAAGT-3′ | Antisense | ||
| hlgA | 170–200 | 5′-ATGATTAAAAATAAAATATTAACAGCAACT-3′ | Sense | 350 | 550 |
| X81586a | 503–527 | 5′-ATCAACATTAGAGTCTTTCGTTTT-3′ | Antisense | ||
| hlgCB | 1904–1926 | 5′-AGCTCTCGAACAACATATTATA-3′ | Sense | 940 | 850 |
| X81586a | 2824–2845 | 5′-CTGCAGCTTTAAGCACTAAAG-3′ | Antisense | ||
| lukED | 1473–1497 | 5′-TAGGCAAATCATCAGTTGCTTCAT-3′ | Sense | 516 | 750 |
| Y13225a | 1967–1989 | 5′-GTAGTTCTGTAACTTTCTTGTTT-3′ | Antisense |
a
Accession number from the EMBL and GenBank libraries.