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. 2022 Jun 18;23(12):6804. doi: 10.3390/ijms23126804

Figure 2.

Figure 2

IL-17 promotes monocyte adhesion in OASFs through upregulating VCAM-1 production. (A) Levels of VCAM-1 were investigated in synovial tissue collected from the GEO database containing OA patients and healthy controls. (B) Spearman’s rank correlation coefficient testing identified significant, positive correlations between VCAM-1 and CD11b. (C–F) IHC staining for VCAM-1 in synovium samples from OA patients and healthy individuals. (G,H) OASFs were stimulated with IL-17 for 24 h, and VCAM-1 synthesis was performed by qPCR and Western blot. (I–M) OASFs were transfected with a VCAM-1 siRNA for 24 h and then treated with or without IL-17 (10 ng/mL). VCAM-1 expression was examined by Western blot and qPCR. Adherent THP-1 cells were photographed and quantified under fluorescence microscopy. Size bar = 320 μm. * p < 0.05 compared with the control group; # p < 0.05 compared with the IL-17-stimulated group.