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. 2022 Jun 18;23(12):6804. doi: 10.3390/ijms23126804

Figure 4.

Figure 4

The JNK pathway mediates IL-17-induced promotion of VCAM-1 synthesis and monocyte adhesion in OASFs. (A,D) OASFs were stimulated with a JNK inhibitor (SP600125, 10 nM) for 30 min or transfected with a JNK siRNA for 24 h and then treated with IL-17 (10 ng/mL) for 24 h. VCAM-1 levels were quantified by qPCR. (B,C,E,F) Adherent THP-1 cells were photographed and quantified under fluorescence microscopy. (G) JNK protein levels were measured by Western blot. (H–J) OASFs were stimulated with IL-17 for the indicated time intervals or pretreated with PKC or JNK inhibitors and then incubated with IL-17 (10 ng/mL). JNK or PKC phosphorylation was performed by Western blot. Size bar = 320 μm. * p < 0.05 compared with the control group; # p < 0.05 compared with the IL-17-stimulated group.