Figure 5.

IL-17 enhances VCAM-1 production by inhibiting miR-5701 expression. (A) Six software databases were examined to predict which miRNAs interfere with VCAM-1 transcription. (B) Levels of miRNAs were investigated in OA and normal, healthy synovial tissue collected from the GEO database. (C–E) OASFs were treated with IL-17 (10 ng/mL). miRNA expression was examined by qPCR. (F,G) OASFs were transfected with miR-5701 mimic and then treated with IL-17 (10 ng/mL). VCAM-1 expression was examined by Western blot and qPCR. (H,I) Adherent THP-1 cells were photographed and quantified under fluorescence microscopy. (J,K) OASFs were stimulated with PKC and JNK inhibitors for 30 min or the respective siRNAs for 24 h and then incubated with IL-17 (10 ng/mL). miR-5701 expression was examined by qPCR. Size bar = 320 μm. * p < 0.05 compared with the control group; # p < 0.05 compared with the IL-17-stimulated group.