TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| E. coli DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rk−mk+) phoA supE44 λ−thi-1 gyrA96 relA1 | BRLb |
| pTrc99A | Expression vector; Ptrc lac1qrrnBT1T2 Ampr | 2 |
| pJB866 | Expression vector; Pm xylS839 oriT Tcr | 4 |
| pEB40 | pUC19 with 5,136-bp EcoRI-SalI DNA fragment containing native phs regulatory and structural genes from S. enterica serovar Typhimurium LT2 | 8 |
| pSB74 | pTrc99A with 5,136-bp EcoRI-SalI DNA fragment containing native phs regulatory and structural genes from S. enterica serovar Typhimurium LT2 | This study |
| pSB77 | pJB866 with 5,136-bp EcoRI-SalI DNA fragment containing native phs regulatory and structural genes from S. enterica serovar Typhimurium LT2 (XhoI-compatible ends) | Bang et al.c |
| pSB103 | pTrc99A with 3,653-bp PCR amplified DNA fragment containing only structural genes of phsA, phsB, and phsC from pSB74 | This study |
| pSB107 | pJB866 with 3,653-bp PCR amplified DNA fragment containing only structural genes of phsA, phsB, and phsC from pSB74 | This study |
a
Abbreviations: Ptrc, hybrid trp-lac promoter; lac1q, gene encoding the lac repressor protein; rrnBT1T2, transcription terminators; Pm, m-cleavage pathway promoter of the TOL plasmid; xylS839, gene encoding the repressor protein that controls Pm; oriT, origin of transfer; Ampr, ampicillin resistant; Tcr, tetracycline resistant; phs, gene for the production of hydrogen sulfide from thiosulfate.
b
BRL, Gibco BRL, Inc., Gaithersburg, Md.
c
S. W. Bang, D. S. Clark, and J. D. Keasling, Abstr. 99th Gen. Meet. Am. Soc. Microbiol. 1999, abstr. Q-302, p. 591, 1999.