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. 2022 Jun 16;23(12):6729. doi: 10.3390/ijms23126729

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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AZA derepresses the PU.1 gene in PU.1^ure/ure AML: (A) methylation of mPU.1 promoter following AZA for 1-week, 3 doses, sets of PU.1^(ure/ure) AML-bearing 5–6 month old mice and control mice were used (n = 4 per group); (B) mRNA expression by qPCR, 1 week AZA treatment in 3 doses, genotypes indicated; (C) Western blot (densitometry) for PU.1 protein in AZA-treated mice with indicated genotypes relative to β-actin; (D) mRNA expression by qPCR (same as B), 1 week AZA treatment in 3 doses, genotypes indicated; (E) flow cytometry of Cd11b expression in PU.1^ure/ure mice, either untreated (light blue) or treated with AZA (violet), or control untreated BM (red); (F) CFU and immature cell colonies (clusters) cultured from c-Kit⁺ cells of control or PU.1^ure/ure mice (n = 3) treated with AZA for 7 days. Mean ± SEM, p-values (t-test, unpaired, two-tailed) * p < 0.05, ** p < 0.05, *** p < 0.005, **** p < 0.0005.

AZA derepresses the PU.1 gene in PU.1^ure/ure AML: (A) methylation of mPU.1 promoter following AZA for 1-week, 3 doses, sets of PU.1^(ure/ure) AML-bearing 5–6 month old mice and control mice were used (n = 4 per group); (B) mRNA expression by qPCR, 1 week AZA treatment in 3 doses, genotypes indicated; (C) Western blot (densitometry) for PU.1 protein in AZA-treated mice with indicated genotypes relative to β-actin; (D) mRNA expression by qPCR (same as B), 1 week AZA treatment in 3 doses, genotypes indicated; (E) flow cytometry of Cd11b expression in PU.1^ure/ure mice, either untreated (light blue) or treated with AZA (violet), or control untreated BM (red); (F) CFU and immature cell colonies (clusters) cultured from c-Kit⁺ cells of control or PU.1^ure/ure mice (n = 3) treated with AZA for 7 days. Mean ± SEM, p-values (t-test, unpaired, two-tailed) * p < 0.05, ** p < 0.05, *** p < 0.005, **** p < 0.0005.