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. 2022 Jun 9;13:924533. doi: 10.3389/fmicb.2022.924533

FIGURE 1.

FIGURE 1

Identification of metabolites packaged in virus particles. (A) Purification of virions. The purity of purified GVE2 virions was examined with transmission electron microscopy. Scale bar, 100 nm. (B) GC-MS analysis of the metabolites extracted from the purified virions and the virus-infected bacteria. Geobacillus sp. E263 was infected by its bacteriophage GVE2. At 1 h post-infection, the bacteria were subjected to compound extraction. The compounds extracted from the purified GVE2 virions and the GVE2-infected E263 were analyzed by GC-MS. Relative abundance of the compounds was plotted with retention time in minute. (C) LC-MS analysis of the metabolites packaged in GVE2 virions. The compounds extracted from the purified GVE2 virions and the virus-infected bacteria were identified by LC-MS. (D) The compounds of GVE2 virions identified by GC-MS. The retention time was indicated in minutes. (E) The compounds of GVE2 virions identified by LC-MS. (F) The GC-MS analysis of the standards and the samples (the compounds from the purified GVE2 virions). The retention time was indicated in minutes. (G) The LC-MS analysis of the standards and the samples (the compounds from the purified GVE2 virions).