Table 1.
Characterization of putative switchgrass laccase proteins. ORF, open reading frame; pI, isoelectric point.
| Gene Name | Gene ID | Number of Amino Acids (aa) | Molecular Weight (kDa) | Signal Peptide Cleavage Site | Signal Peptide Length | ORF Length | pI |
|---|---|---|---|---|---|---|---|
| PvLAC1 | Pavir.1KG385700 | 581 | 63.6 | VAA-TP | 24 | 1746 | 6.76 |
| PvLAC2 | Pavir.1NG538200 | 581 | 64.0 | VAA-SP | 24 | 1746 | 6.39 |
| PvLAC3 | Pavir.1NG327600 | 575 | 63.0 | AQA-LR | 24 | 1728 | 6.62 |
| PvLAC4 | Pavir.2KG328400 | 570 | 62.4 | ARA-AT | 25 | 1713 | 6.95 |
| PvLAC5 | Pavir.2KG017500 | 569 | 62.1 | ADA-AV | 24 | 1710 | 8.55 |
| PvLAC6 | Pavir.2NG308200 | 566 | 62.4 | ARA-AT | 24 | 1701 | 6.81 |
| PvLAC7 | Pavir.2NG307700 | 568 | 62.4 | ARA-AT | 20 | 1706 | 8.84 |
| PvLAC8 | Pavir.3KG084800 | 619 | 68.5 | TMA-LP | 25 | 1860 | 7.09 |
| PvLAC9 | Pavir.3KG123800 | 560 | 61.2 | AEA-KV | 19 | 1683 | 6.51 |
| PvLAC10 | Pavir.3KG266100 | 530 | 58.2 | / | / | 1593 | 5.19 |
| PvLAC11 | Pavir.3NG041300 | 563 | 62.1 | TMA-LP | 22 | 1692 | 6.44 |
| PvLAC12 | Pavir.4NG327800 | 619 | 67.1 | / | / | 1860 | 8.98 |
| PvLAC13 | Pavir.4NG191400 | 585 | 63.0 | AEA-ET | 32 | 1758 | 6.92 |
| PvLAC14 | Pavir.4NG191000 | 579 | 62.6 | AAA-RT | 29 | 1740 | 9.78 |
| PvLAC15 | Pavir.5KG622300 | 578 | 62.9 | AEG-AI | 24 | 1737 | 7.64 |
| PvLAC16 | Pavir.5KG622200 | 582 | 63.3 | VQG-IT | 28 | 1749 | 8.83 |
| PvLAC17 | Pavir.5KG610700 | 569 | 62.7 | AGA-EV | 22 | 1710 | 5.90 |
| PvLAC18 | Pavir.5KG623300 | 580 | 63.4 | AAG-DT | 34 | 1743 | 9.05 |
| PvLAC19 | Pavir.5KG629400 | 649 | 67.6 | ADA-AT | 16 | 1950 | 5.81 |
| PvLAC20 | Pavir.5KG629500 | 535 | 58.0 | / | / | 1608 | 5.95 |
| PvLAC21 | Pavir.5KG634700 | 570 | 60.7 | AHA-AT | 18 | 1713 | 5.82 |
| PvLAC22 | Pavir.5KG634800 | 647 | 67.2 | ADA-AT | 16 | 1944 | 6.10 |
| PvLAC23 | Pavir.5KG007300 | 549 | 60.6 | VSS-AE | 22 | 1650 | 9.63 |
| PvLAC24 | Pavir.5NG584800 | 578 | 63.0 | TEG-AI | 24 | 1737 | 7.21 |
| PvLAC25 | Pavir.5NG584700 | 584 | 63.4 | VQG-IT | 29 | 1755 | 8.73 |
| PvLAC26 | Pavir.5NG569300 | 569 | 62.8 | AGA-EV | 22 | 1710 | 5.97 |
| PvLAC27 | Pavir.5NG585100 | 573 | 62.8 | AAG-DT | 27 | 1722 | 8.87 |
| PvLAC28 | Pavir.5NG053100 | 589 | 65.5 | / | / | 1770 | 6.60 |
| PvLAC29 | Pavir.6KG211600 | 516 | 55.8 | / | / | 1551 | 6.24 |
| PvLAC30 | Pavir.7NG016200 | 564 | 62.2 | GHA-AT | 18 | 1695 | 8.76 |
| PvLAC31 | Pavir.8KG104100 | 637 | 68.7 | / | / | 1914 | 9.77 |
| PvLAC32 | Pavir.8KG112100 | 567 | 62.3 | GRA-AI | 19 | 1704 | 9.05 |
| PvLAC33 | Pavir.8KG177500 | 573 | 61.9 | VVA-KE | 25 | 1722 | 6.43 |
| PvLAC34 | Pavir.8KG103400 | 583 | 62.8 | AEA-ET | 29 | 1752 | 7.67 |
| PvLAC35 | Pavir.8KG057100 | 563 | 61.5 | / | / | 1692 | 5.72 |
| PvLAC36 | Pavir.8KG368500 | 576 | 63.3 | AMA-AV | 28 | 1731 | 5.86 |
| PvLAC37 | Pavir.8KG368600 | 587 | 64.7 | GEA-AV | 22 | 1764 | 6.04 |
| PvLAC38 | Pavir.8NG320100 | 589 | 65.0 | GEA-AV | 24 | 1770 | 6.33 |
| PvLAC39 | Pavir.8NG328000 | 596 | 65.2 | AAG-AV | 28 | 1791 | 6.43 |
| PvLAC40 | Pavir.8NG353900 | 565 | 61.4 | VVA-KE | 22 | 1698 | 6.62 |
| PvLAC41 | Pavir.8NG327600 | 621 | 69.3 | AVA-AS | 24 | 1866 | 6.13 |
| PvLAC42 | Pavir.8NG327500 | 589 | 65.3 | GEA-AV | 24 | 1770 | 6.11 |
| PvLAC43 | Pavir.8NG321100 | 617 | 68.8 | AVA-AS | 23 | 1854 | 6.10 |
| PvLAC44 | Pavir.8NG003600 | 565 | 61.4 | VVA-KE | 22 | 1698 | 6.62 |
| PvLAC45 | Pavir.9KG472600 | 607 | 66.0 | ALA-EE | 28 | 1824 | 8.58 |
| PvLAC46 | Pavir.9KG493900 | 571 | 62.3 | SHG-AT | 22 | 1716 | 8.64 |
| PvLAC47 | Pavir.9NG438800 | 585 | 63.0 | AEA-ET | 32 | 1758 | 6.92 |
| PvLAC48 | Pavir.9NG711200 | 601 | 65.3 | ALA-EE | 26 | 1806 | 8.43 |
| PvLAC49 | Pavir.9NG700300 | 571 | 62.3 | SHG-AT | 22 | 1716 | 8.74 |