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. 2022 Jun 7;23(12):6385. doi: 10.3390/ijms23126385

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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M1 and M2 oxidative stress comparison. (a) mRNA relative expression (2^−ΔCt) of NOX2 and SOD2 involved in the regulation of oxidative stress has been studied by RT-qPCR in M1 (green) and M2 (red) macrophages and normalized with 18S expression; (b) graph representing the percentage of ROS positive in M1 (green) and M2 (red) macrophages, plots show the histogram of gated cells with the 2 populations: ROS(−) (blue) and ROS(+) (red) cells; (c) immunofluorescence of Nrf2 (green) expression in M1 and M2 macrophages. Nuclei were stained with DAPI (blue) (scale = 50 µm). ** = p ≤ 0.01; *** = p ≤ 0.001.