Table 3.
Immunofluorescence conditions regarding primary antibodies.
| Targets | Antibodies | Blocking Solutions | Primary Antibody Dilution and Incubation | Secondary Antibodies |
|---|---|---|---|---|
| CD68 | Anti-human, rabbit monoclonal, anti-CD68, Cell Signaling | PBS/NGS 5%/Triton 0.3% (1 h) | 1/800, overnight 4 °C | Goat anti-Rabbit IgG (H + L) Highly Cross-Absorbed Secondary Antibody, Alexa Fluor Plus 488 |
| CD14 | Anti-human, mouse monoclonal, anti-CD14, Miltenyi Biotec | PBS/BSA 2% (20 min) | 1/100, overnight 4 °C | Goat anti-Mouse IgG (H + L) Highly Cross-Absorbed Secondary Antibody, Alexa Fluor Plus 555 |
| CD36 | Anti-human, rabbit monoclonal, anti-CD36, ThermoFisher Scientific | PBS/BSA 2% (20 min) | 1/100, overnight 4 °C | Goat anti-Rabbit IgG (H + L) Highly Cross-Absorbed Secondary Antibody, Alexa Fluor Plus 488 |
| CD80 | Anti-human, mouse monoclonal, anti-CD80, R&D Systems | PBS/casein 0.5% (1 h) | 1/50, overnight 4 °C | Goat anti-Mouse IgG (H + L) Highly Cross-Absorbed Secondary Antibody, Alexa Fluor Plus 555 |
| CD86 | Anti-human, rabbit monoclonal, anti-CD86, Cell Signaling | PBS/NGS 5%/Triton 0.3% (1 h) | 1/100, overnight 4 °C | Goat anti-Rabbit IgG (H + L) Highly Cross-Absorbed Secondary Antibody, Alexa Fluor Plus 488 |
| CD163 | Anti-human, mouse monoclonal, anti-CD163, Sanbio | PBS/BSA 2% (20 min) | 1/50, overnight 4 °C | Goat anti-Mouse IgG (H + L) Highly Cross-Absorbed Secondary Antibody, Alexa Fluor Plus 555 |
| CD206 | Anti-human, mouse monoclonal, anti-CD206, Miltenyi Biotec | PBS/BSA 2% (20 min) | 1/100, overnight 4 °C | Goat anti-Mouse IgG (H + L) Highly Cross-Absorbed Secondary Antibody, Alexa Fluor Plus 555 |
| Nrf2 | Anti-human, rabbit monoclonal, anti-Nrf2, Cell Signaling | PBS/NGS 5%/Triton 0.3% (1 h) | 1/200, overnight 4 °C | Goat anti-Rabbit IgG (H + L) Highly Cross-Absorbed Secondary Antibody, Alexa Fluor Plus 488 |