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. 2022 Jun 20;23(12):6875. doi: 10.3390/ijms23126875

Figure 6.

Figure 6

Characterization of reverted BCAFs. (A,B) Representative images of the outgrowth and spreading of reverted BCAFs from BCAF spheroids transferred on a conventional 2D cell culture dish to allow 2D cell growth and proliferation after 216 h of 3D cell culture on agar. Reverted BCAFs outgrowing from spheroid after (A) 1 day and (B) 1 week of 2D cell growth. (C) Representative cell morphology of reverted BCAFs by phase-contrast microscopy. Scale bar 200 µm. Magnification ×10. (D–I) Western blotting analysis of α-SMA, COX-2 and vimentin in protein extracts of BCAF monolayers (ML) and reverted BCAFs (REV). GAPDH was used as loading control. Representative images of at least three independent experiments are shown. (G–I) Densitometric analyses of α-SMA, COX-2 and vimentin protein levels. Data are reported as means of at least three independent experiments ± S.E. * p < 0.05.