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. 2022 Jun 10;23(12):6516. doi: 10.3390/ijms23126516

Figure 3.

Figure 3

Effect of AZA treatment in three human BUC cells. (A) Frequency of methylation of CLDN4 promoter in T24, RT4, and UMUC3 human BUC cells with or without AZA treatment. (B) Effect of AZA on mRNA expression. (C) Effect of AZA on CLDN4 protein expression and tight junction integrity in T24 cells. (D) Effect of AZA on TER in T24 cells. Error bar indicates SD calculated by Student’s t-test. CLDN, claudin; BUC, bladder urothelial carcinoma; AZA, 5-aza-2-deoxycytidine; SDS-CLDN4, 0.5% sodium dodecyl sulfate-solubilized CLDN4 (whole CLDN4); T20-CLDN4, 0.5% Tween 20-solubilized CLDN4 (tight junction unintegrated CLDN4); TER, transepithelial electrical resistance; CB, cytochalasin B.