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. 2022 Jun 10;23(12):6516. doi: 10.3390/ijms23126516

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of CLDN4 knockdown in three human BUC cells. (A) Effect of CLDN4 knockdown on mRNA expression. (B) Effect of CLDN4 knockdown on sphere formation. (C) Effect of CLDN4 knockdown on CLDN4 expression and tight junction integrity. (Right) Semi-quantification of CLDN4 protein levels in siCL4-treated T24 cells. (D) Effect of CLDN4 knockdown on TER in T24 cells. Error bar indicates SD calculated by Student’s t-test. CLDN, claudin; BUC, bladder urothelial carcinoma; si, short interference RNA; siC, control siRNA; siCL4, siRNA for CLDN4; SDS-CLDN4, 0.5% sodium dodecyl sulfate-solubilized CLDN4 (whole CLDN4); T20-CLDN4, 0.5% Tween 20-solubilized CLDN4 (tight junction unintegrated CLDN4); ppt, immunoprecipitant; TER, transepithelial electrical resistance; CB, cytochalasin B.