(A) Relative activity of native xanthan lyase (purified, open symbols) and recombinant xanthan lyase (cell extract, closed symbols) at different pH values, on xanthan in McIlvaine buffer. (B) Relative activity of native xanthan lyase (purified, open symbols) and recombinant xanthan lyase (cell extract, closed symbols) at different temperatures, on xanthan in 15 mM potassium phosphate buffer, pH 7. The activity of both enzymes was set at 100% at the conditions under which the native xanthan lyase was most active. T, temperature.