TABLE 1.

Amino acid sequences of the N terminus and internal peptides of purified pyruvated mannose-specific xanthan lyase from P. alginolyticus XL-1 and nucleotide sequences of the derived degenerate primers

Peptide	Sequencea	Degenerate oligonucleotide primerb
N terminus	SDEYDTLRLKWRDM (residues 1–14)	D2: 5′-GAY GAR TAY GAY ACV TTR C-3′
15	SXISSENSIGT (residues 324–334)	D5: 5′-TCS GAR AAY TCS ATY GGH AC-3′; D7c: 5′-GTD CCR ATS GAR TTY TC-3′
17	TSAQVSSYASNPNISVL (residues 626–642)	D12: 5′-CTR ATR TTN GGR TTR CTN GC-3′
25	TPGGTTNYLXVDLR (residues 49–62)
28	ENTLNVVGVNFX (residues 653–664)

^aX can be either cysteine or tryptophan. Cysteine was not determined with the method used. Tryptophan was masked by diphenylthiourea, which is formed from phenylisothiocyanate in the Edman degradation. Underlined amino acids were used to design degenerate primers. Residue numbers refer to the position in the deduced amino acid sequence of the mature enzyme encoded by the xalA gene (1 = the N-terminal residue). 

^bD = (A + T + G); H = (A + T + C); N = (A + C + T + G); R = (A + G); S = (C + G); V = (A + C + G); Y = (C + T). 

^cPrimer D7 is complementary to D5.