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The designed primer sequence by comparing.
| Primer Name | Primer Sequence (5′—3′) |
|---|---|
| EaCent-F | CGGTTTGTTTGGAGACTTGC |
| EaCent-R | GCCCTAAATGATTTCTGAGCCTAT |
| EaST1-F | TTTTGGGACTCAGTTTCATTTC |
| EaST1-R | TGAAGACGCTAGAGTAGTATTTGTG |
| EaST2-F | TTACCATAAGCCACAAATC |
| EaST2-R | CATCTAAATACTCCACCCTAACT |
