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. 2022 Jun 15;23(12):6660. doi: 10.3390/ijms23126660

Figure 3.

Figure 3

Effect of IL-17 on DKK1 expression and WNT pathway activation. DKK1 and Axin2 expression levels in the control group and AS group were determined by the quantitative RT-PCR method. (a) DKK1 relative expression following cell culture in expansion medium in the presence (IL-17) or absence (UT) of 50 ng/mL IL-17 for 7 days (EM 7 days) and (b) in osteogenic medium in the presence (IL-17) or absence (UT) of 50 ng/mL IL-17 for 21 days (OM 21 days). (c) Wnt activation marker Axin2 expression levels following cell culture in expansion medium in the presence (IL-17) or absence (UT) of 50 ng/mL IL-17 for 7 days (EM 7 days) and (d) in osteogenic medium in the presence (IL-17) or absence (UT) of 50 ng/mL IL-17 for 21 days (OM 21 days). All graphs indicate mean ± SD. Each assay is expressed as fraction of the UT expression normalized to unity. * = p < 0.05; ** = p < 0.01; *** = p < 0.001; **** = p < 0.0001.