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. 2022 Jun 15;23(12):6660. doi: 10.3390/ijms23126660

Figure 5.

Figure 5

IL-17 induced mineralization in MSC cell cultures from AS group. MSCs were cultured in osteogenic medium (OM) for 21 days in the presence (IL-17) or absence (UT) of 50 ng/mL IL-17. (a) Alizarin red staining of AS cell cultures; macroscopic photographs (top row) and microscope images (bottom row). (b) Scatterplots depicting the quantification of Alizarin red stain density for AS and control group cell cultures. (c) Von Kossa staining of AS cell cultures; macroscopic photographs (top row) and microscope images (bottom row). (d) Densitometry analysis of Von Kossa stain. Mean ± SD. * = p < 0.05; *** = p < 0.001.