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. 2022 Jun 14;23(12):6636. doi: 10.3390/ijms23126636

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Confocal microscope images showing laser-induced modifications in three different types of microfibres. (A) Sharp channel inside a dehydrated microfibre after spending 41 min on a sample holder. (B) Attenuation (blue circle) of signal density inside a hydrated microfibre after 9 min spent on a sample holder. After being marked with Live/Dead^® ki, A and B colors were modified to highlight laser interaction effects. (C) Sharp channel inside a PFA-fixed microfibre, green: collagen-Fluorescein-5-isothiocyanate (FITC), blue: DAPI staining.