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. 2022 Jun 7;23(12):6397. doi: 10.3390/ijms23126397

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Transmission electron micrographs revealed significant increases in the numbers of autophagic vacuoles (arrows) in granulosa cells from females treated with low (A) and high doses of allethrin (B,C). Autophagic vacuoles are double-membrane structures that contain a number of atypical and/or injured mitochondria, endoplasmic reticulum membranes and cytoplasm (C). However, these autophagosomes were not detected in oocytes or theca cells. GC: granulosa cell, Nu: nucleus, Op: ooplasm, TC: theca cell and ZP: zona pellucida.