Table 3.
Recent works in single-cell proteomics.
| Technique | Description | Research | Reference |
|---|---|---|---|
| Single-cell IsoCode chip | Highly multiplexed chip with an antibody barcode array. Simultaneous detection of secreted proteins from individual cells. | They deciphered functional heterogeneity among patients and predicts clinical response and toxicities of CAR products. | Liu, D., et al. [60] |
| Chimeric Antigen Receptor (CAR) | Single-pass transmembrane receptor to target cancer cells, achieving a high rate of remission. Mainly used against hematological malignancies | CD19-CAR T cell stimulation activated CD19-CAR-specific pathways and canonical TCR signaling. | Griffith, A. A., et al. [61] |
| Quantification of RNA and intracellular epitopes by sequencing (QuRIE-seq) | High-throughput droplet-based platform to quantify single-cell RNA and (phospho)protein by sequencing within thousands of single cells. | They identified cell-state changes at signaling and transcriptome level after stimulation of the B-cell receptor pathway in Burkitt’s lymphoma cells. | Rivello, F., et al. [67] |
| Deep Visual Proteomics (DVP) | Image analysis of individual tumor cells based on artificial intelligence combined with single-cell/nucleus laser microdissection and ultra-high-sensitivity MS. | Changes in the proteome on melanocytes progressing to melanoma were characterized, uncovering pathways that spatially vary as the cancer progresses. | Mund, A., et al. [69] |
| Single Cell ProtEomics by Mass Spectrometry (SCoPE2) | An automated and miniaturized sample preparation workflow to increase quantitative accuracy and throughput while lowering cost and time. | Exploration of monocytes differentiation into macrophage-like cells uncovered a gradient of proteome states in the absence of polarizing cytokines. | Specht, H., et al. [41] |
| Single-pot Solid-phase enhanced Sample preparation (SP3) | An approach using functionalized paramagnetic beads to trap peptides within a magnetic field, optimizing the sample amount needed while maximizing analyte recovery. | Single-cell analysis of the human oocytes’ proteome identified differential protein expression and fundamental preservation of the genome integrity during maturation. | Virant-Klun, I., et al. [24] |