Figure 4.

Polyamine metabolism is influenced by SBP-101 treatment through ODC inhibition and modest SSAT upregulation. Following treatment with 10 μM of BENSpm or SBP-101 for 24 h, lung, pancreatic, and ovarian adenocarcinoma cells were collected, and lysates were tested for ODC and SSAT enzyme activity. Both BENSpm and SBP-101 substantially reduced the activity of the biosynthetic enzyme ODC (A). BENSpm treatment resulted in the induction of the catabolic enzyme SSAT in all treated cell lines. SBP-101 also upregulated SSAT activity, albeit to lower levels than BENSpm treatment (B).