Table 2.
Performance of kinetic models to describe the thermal inactivation of lipases from B. cepacia (Lipase PS) and R. miehei (Palatase).
| Lipase | Model (Eq.) | r2 | χ2 | SEM | Remark |
|---|---|---|---|---|---|
| Lipase PS | First-order (1) | [0.971;0.996] | [0.0004;0.0030] | [0.0024;0.0170] | Accepted: high r2 and low SEM and χ2; good fit for dependence temperature parameters |
| Weibull (2) | [0.973;0.996] | [0.0004;0.0325] | [0.0024;0.1819] | Rejected: n = 1 (first-order model) | |
| Distinct isoenzymes (3) | [0.974;0.997] | [0.0004;0.0068] | [0.0025;0.0383] | Rejected: negative parameter estimates | |
| Two-fraction (4) | [0.971;0.997] | [0.0004;0.0078] | [0.0020;0.0437] | Rejected: negative parameters estimates | |
| Multi component first order (5) | [0.974;0.996] | [0.0006;0.0082] | [0.0034;0.0414] | Rejected: negative parameters estimates | |
| Series a (6) | — | — | — | Rejected: not generate parameters answers, either coefficients of determination | |
| nth order (7) | — | — | — | Rejected: not generate parameters answers, either coefficients of determination | |
| Fractional conversion (8) | — | — | — | Rejected: not generate coefficients of determination | |
| Palatase | First-order (1) | [0.687;0.833] | [0.0452;0.1601] | [0.2145;0.6537] | Rejected: low r2 and high SEM and χ2 |
| Weibull (2) | [0.965;0.994] | [0.0011;0.0026] | [0.0017;0.0124] | Accepted: higher r2 and lower SEM and χ2 | |
| Distinct isoenzymes (3) | [0.749;0.872] | [0.0204;0.0425] | [0.1087;0.2144] | Rejected: equal parameter estimates; kL = kR | |
| Two-fraction (4) | [0.896;0.946] | [0.0106;0.0376] | [0.0536;0.4599] | Rejected: negative parameters estimates | |
| Multi component first order (5) | [0.896;0.946] | [0.7850;1.6388] | [1.3653;8.2733] | Rejected: negative parameters estimates | |
| Series a (6) | — | — | — | Rejected: not generate coefficients of determination | |
| nth order (7) | — | — | — | Rejected: not generate parameters answers, either coefficients of determination | |
| Fractional conversion (8) | — | — | — | Rejected: not generate coefficients of determination |
a Assuming α2 =0, the final form of the enzyme is totally deactivated.