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Nurr1 siRNA knockdown does not alter BDNF gene expression. Primary cortical cell cultures were treated with 35 pmol scrambled siRNA or Nurr1 siRNA and 48 h later were harvested for real-time qRT-PCR. (a) Compared to the control group, Nurr1 mRNA was reduced significantly in the siNurr1 group. (b) BDNF mRNA remained unchanged between groups. Two-way ANOVA and post hoc independent samples t-test, *** p < 0.001, n = 5 per group. Error bars: ±SE.
