Figure 4.

Nurr1 knockdown does not affect activity-dependent BDNF gene expression. Primary cortical neurons were treated with 50 mM KCl and 35 pmol of scrambled siRNA or Nurr1 siRNA. Cells were harvested forty-eight hours post-transfection and underwent harvest for real-time qRT-PCR. (a) Nurr1 mRNA was reduced significantly in the siNurr1 group compared to the control group. (b) BDNF mRNA remained unchanged between groups. *** p < 0.0001, two-way ANOVA and post hoc independent samples t-test (n = 5–6 per group). Error bars: ±SE.