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. 2022 Jun 9;13:918112. doi: 10.3389/fpls.2022.918112

Figure 5.

Figure 5

Coupling of RNA-seq, BSA, and QTL mapping to identify auxin genes functioning in leafy head formation. GO (A) and KEGG pathway (B) analysis of DEGs from the RNA-seq data of Ab/Ad tissue of curling leaf veins (see materials and analysis flow chart in Supplementary Figures S3D,F, S5A). (C) Venn diagrams for the DEGs of different comparisons of different Brassica rapa crops during the farming season (see materials and analysis flow chart in Supplementary Figures S3E, S5E). EHG, the DEGs of early-heading Chinese cabbage; LHG, the DEGs of late-heading Chinese cabbage; NH, the DEGs of the non-heading pak choi materials. (D) The ΔSNP index values (95% confidence interval) used for the association analysis of heading. (E,F) Overlapped intervals identified by QTL mapping. The vertical axis indicates LOD-scores; The horizontal axis shows genetic positions; The LOD-score threshold of 3 is indicated by the horizontal dashed line. DEG, differentially expressed gene; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; BSA, bulk segregant analysis; LOD, Logarithm of odds. Ad/Ab, adaxial/abaxial.