Table 1.
Experimental outline of a single biological replicate to investigate the effect of covered vs. uncovered exposure tubes and number of mosquitoes per test unit. The test concentration was 0.043% for Anopheles gambiae (Kisumu, susceptible) testing and 0.75% for An. gambiae (Tiassalé 13, resistant) testing. Mosquito age was 2–5 days for both strains and all treatments.
| Test Unit | Treatment | Number per Test Unit | Covered/Uncovered |
|---|---|---|---|
| Negative Control 1 | Silicone oil only | 25 | Uncovered |
| Negative Control 2 | Silicone oil only | 25 | Uncovered |
| Uncovered 1 | Permethrin | 25 | Uncovered |
| Uncovered 2 | Permethrin | 25 | Uncovered |
| Covered 1 | Permethrin | 25 | Covered |
| Covered 2 | Permethrin | 25 | Covered |
| 30 per test unit 1 | Permethrin | 30 | Uncovered |
| 30 per test unit 2 | Permethrin | 30 | Uncovered |
| 20 per test unit 1 | Permethrin | 20 | Uncovered |
| 20 per test unit 2 | Permethrin | 20 | Uncovered |
| 15 per test unit 1 | Permethrin | 15 | Uncovered |
| 15 per test unit 2 | Permethrin | 15 | Uncovered |
| 10 per test unit 1 | Permethrin | 10 | Uncovered |
| 10 per test unit 2 | Permethrin | 10 | Uncovered |