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. 2022 Jun 1;10(2):21. doi: 10.3390/jdb10020021

Figure 5.

Figure 5

Trunk spinal cord ependymal cells represent a heterogeneous population with distinct GFAP+ and HuCD+ populations. (A) HuCD+ cells are present within the trunk spinal cord and are in close contact with the central canal. HuCD+ cells have either weak (filled arrow) or absent NeuN expression (open arrows). Note that NeuN expression in HuCD+/NeuN+ cells does not reach the intensity of NeuN expressing neurons of the grey matter (inset). (B) The ependymal layer of the trunk spinal cord contains distinct populations of GFAP+/HuCD–cells (open arrows) and GFAP–/HuCD+ cells (filled arrows). GFAP+ cells reside peripherally in the ependymal layer. (C–E) GFAP/HuCD expression in the ependymal layer of the trunk spinal cord before (C), 2 days (D) and 8 days (E) post-autotomy. Note that expression remains relatively constant across time points. (F) The original tail spinal cord contains distinct GFAP+/HuCD– and GFAP–/HuCD+ populations of ependymal cells. The locations at which these cells reside in the tail mirror what is found in the trunk spinal cord. Representative sections from cranial trunk spinal cord was used for all images. dpa = days post-autotomy. Scale bar: (A–F) = 10 µm.