TABLE 2.

Summary of primers

Primer	Sequence (5′-3′)a	Location (nucleotides)	Comment
WG-1	CCYTCRCGRCCGCCCCA	1913–1897b	L. lactis B-4449 xylA PCR
NH-1	ACNAARACNAARAAYATGTTY	1378–1399b	L. lactis B-4449 xylA PCR
5	TGATGCCAATCTTGG	2220–2234b	L. lactis B-4449 xylA inverse PCR
7	TCTGGTAGGTCTTCTCCTA	∼1.6 kg upstream of xylA	L. lactis B-4449 xylA inverse PCR
BFX	CGGGATCCATGTTCATTCTCTCCTAATCAC	1171–1192c	xylA PCR and Northern primer
HRX	CCCAAGCTTGGTTCCTAAGT	2702–2691c	xylA PCR and Nothern primer
BFX-rev	GTGATTAGGAGAGAATGAACAT	1192–1171c	xylR-xylB inverse PCR
HRX-rev	TTAGGAATTGACTTAGGAACC	2681–2702c	xylR-xylB inverse PCR
210ip820-rev	TGAGTAACAAGCACCCGGAACC	3544–3523c	xylB inverse PCR
R210xb-rev	TAGAAGCGGTCACTAAAGC	4011–4029c	xylB inverse PCR
Ip412-rev	AGAAATTGCTGGATAAACTGG	2964–2945c	xylB inverse PCR
M13-40	GTTTTCCCAGTCACGAC	NAd	Vector sequencing primer
M13rev(ht)	GCTTTAGTGACCGCTTCTA	NA	Vector sequencing primer

^aR = A or G; Y = C or T; H = A or C or T; N = any nucleotide. 

^bNubmering corresponds to L. lactis B-4449 xyl-xyn operon (including sequence upstream of xylR); GenBank accession no. AF092042. 

^cNumbering corresponds to L. lactis IO-1 xyl-xyn operon (including upstream sequence); GenBank accession no. AF092041. 

^dNA, not available.