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C2C12 myotubes were immunostained with myosin heavy chain (MyHC; red), and the nuclei were immunostained with DAPI (blue). Scale bar: 100 μm. Representative images were taken (A). Two independent experiments (n = 7) showed the same trend in myotube diameter (B), myotube area (C), differentiation index (D), and Multinucleation (E). * p < 0.05 vs. con; ** p < 0.01 vs. con.
