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Representative nested-PCR agarose gel of the DNA extracted from soil samples, demonstrating 16 amplified fragments with the amplification of 152 bp fragments. 1 and 24 = Molecular Weight (1 kb Plus–Invitrogen), 2 to 10 = samples from Seropédica, and 11 to 19 = samples from Nova Iguaçu, 20 = Positive control S. brasiliensis (IPEC 16490) and 21, 22 and 23 = Negative control.
