Figure 6.

Changes in biosynthesis processes in early-stage MAFLD. (A) The log2 fold changes of protein abundance in alb-SREBP-1c vs. C57Bl6 hepatocytes (n = 6 animals per condition). 2731 differentially abundant proteins were detected. Upregulated (red; n = 46) or downregulated proteins (blue; n = 26) were determined by Student’s t-test, with criteria set to p-value < 0.05 and >1.5-fold regulation. Proteins regulated by alb-SREBP-1c were subjected to functional enrichment analyses. Significantly enriched pathways (FDR < 0.1) are shown for biological processes (GO). A modified Fisher’s exact test was used for the functional enrichment analyses. p-values are corrected for multiple testing using the Benjamini–Hochberg (FDR) method. (B–D) Heat maps resulting from z-score analyses and estimation plots for each pathway are included: (B) de novo lipogenesis, (C) fatty acid import/transport, (D) fatty acid β-oxidation (mitochondrial). Estimation plots show the differences between means of alb-SREBP-1c vs. C57Bl6 (±95% CI) (mean z-score: left axis, effect size: right axis). Functional analysis show data expressed as means (±95% CI, n = 3–6 per group—depending on the assay). Color code for assays (B–D): white bars, data from C57Bl6 mice and gray bars, data from alb-SREBP-1c mice. Statistics: Mann–Whitney test or two-way ANOVA with Tukey’s multiple comparisons, ** p < 0.01, **** p < 0.0001. C57: C57Bl6, alb1c: alb-SREBP-1c.