TABLE 2.

Different rDNA IGS types and restriction patterns determined by PCR-RFLP analysis of the rDNA IGS regions

rDNA IGS clusters and their composition	rDNA IGS typea	Sizeb of IGS PCR product (bp)	Different restriction patterns typesc of rDNA IGS digested with:
			AluI	DdeI	HaeIII	HhaI	HinfI	MspI	NdeII	RsaI
Cluster I	1	825	2	2	2	31	3	2	2	2
	2	824	2	2	2	3	3	2	2	2
	3	820	2	2	2	4	3	2	3	2
	4	823	2	2	2	2	3	2	2	3
	5	816	2	2	2	3	3b	2	2	8
	6	802	2	2	2	31	2	2	2	5
	7	820	2	2	2	2	14	2	4	2
	8	825	2	2	2	2	2	2	2	2
	9	825	2	2	2	3	2	2	4	2
Cluster II	10	821	2b	2	2	28	2	9b	27b	17
B. japonicum	11	825	22	23	9	28	2	9b	27b	17
Cluster III	13	900	4	22	2	3	16	20	5	6
Cluster IV	18	820	4	22	30	11	21	35	3	5
	19	850	4	22	30	11	2	36	37	5
Cluster V	24	850	12	6	9	13	6	10	18	7
Bradyrhizobium sp. (F. albida)	25	854	12	19	9	2	6	10	18	7
B. japonicum	26	820	12	19	9	2	6	10	16	7
	27	860	12	6	9	11	6	10	18	8
	28	850	12	6	36	11	2	10	18	8
	29	864	12	6	10	2	6	10	18	7
	30	853	12	6	10	32	6	10	18	7
	31	900	12	6	10	2	2	10	18	7
	32	900	12	6	10	39	2	10	18	8
Cluster VI	33	804	11	6	34	40	2	29	15	5
	34	862	11	6	11	12	5	11	15	6
	35	862	29	6	11	5	5	29	31	2
Cluster VII	36	870	10	6	9	11	2	12	17	5
B. japonicum	37	880	10	6	9	11	6	12	17	6
Cluster VIII	38	900	7	3	5	23	5	6	7	5
	39	920	7	3	5	6b	6	6	7	6
	40	913	7	3b	6	6	6	6	7	6
	41	842	7	3	5	23	19	5b	7b	4
Bradyrhizobium sp. (A. elaphroxylon)	42	950	8	3	5	7	19	5	7	4
Cluster IX	44	780	5	2	15	45	2	10	4	5
B. liaoningense
Cluster X	45	845	16	7	12	14	5	13	14	10
Bradyrhizobium sp. (F. albida)
Cluster XI	46	850	15	20	14	20	5	15	13	12
	47	824	13	21	14	34	5	15	15	12
Bradyrhizobium sp. (A. mangium)	48	843	13	21	14	18	6	15	12	12
Bradyrhizobium sp. (A. mollissima)	49	862	14	10	15	18	6	17	12	11
Bradyrhizobium sp. (F. albida)	50	864	14	20	14	18	6	16	12	11
B. elkanii	51	860	14	20	15	8	2	16	12	11
	52	870	15	20	14	18	5	15	13	11
	53	852	23	10	15	18	6	17	12	11
Cluster XII	54	822	19	8	13	16	6	14	22	11
Bradyrhizobium sp. (F. albida)	55	800	19	8	13	15	6	14	22	11
Cluster XIII	57	866	21	4	7	8	7	7	9	6
	58	850	20	4	8	9	7	7	10	6
Bradyrhizobium sp. (F. albida)	59	840	20	4	8	9	7	8	10	6
	60	840	20	4	8	9	7	8	10	5
Cluster XIV	61	880	21	5	8	9	6	9	11	6
Bradyrhizobium sp. (F. albida)	62	862	21	5	8	31	6	9	11	6
Cluster XV	63	1,000	9	14	22	25	12	22	8	15
Bradyrhizobium sp. (A. indica)	64	1,000	9	14	23	25	12	23	8	15
Cluster XVI	66	1,038	17	11	19	22	10	19	19	13
Bradyrhizobium sp. (A. afraspera)	67	950	17	11	18	21	9	19	20	18
Separate	12	780	2	2	31	2	2	2	34	2
	14	795	3	2	2	37	2	2	4	21
	20	870	31	2	30	23	5	20	26	5
	21	832	4	28	32	31	21	31	3	20
	22	900	34	13	2	7	5	33	37	13
	23	920	32	29	2	23	23	26	35	13
	56	880	13	20b	30	15	6	35	38	23
	70	923	25	17	26	20	10	26	28	13
Bradyrhizobium sp. (A. decurens)	15	814	3	2	3	5	4	2	3	3
B. japonicum	16	840	37	33	3	20	4	9	18	2
Bradyrhizobium sp. (L. luteus)	17	806	5	23	3	28	4	3	3	5
Bradyrhizobium sp. (A. afraspera)	43	913	27	15	24	7	14	24	26	16
Bradyrhizobium sp. (A. sensitiva)	65	1,006	28	25	27	30	12	28	30	15
Bradyrhizobium sp. (A. sensitiva)	68	934	26	12	21	23	13	21	23	6
Bradyrhizobium sp. (F. albida)	69	910	25	9	7	17	8	2	29	13

^aAn rDNA IGS type corresponds to one combination of restriction patterns obtained with eight restriction enzymes. 

^bThe size of the PCR product corresponds to the mean size of the undigested PCR product estimated by summing the sizes of the restriction fragments. Results of Bradyrhizobium strains from small legumes are in bold. 

^cA restriction pattern type refers to the pattern obtained for one strain with one restriction enzyme.