Figure 3.

Time-dependent average pH and histogram of pH distribution on hyphal surfaces of C. cinerea. The pH was assessed by the hyphal-bound Synechocystis sp. PCC6803 bioreporter cells. (a) Bioreporter data encompass the average and standard deviation of n > 2400 cells (circles, (a)). Experiments in the absence of C. cinerea served as controls (triangles; n > 2200 cells). Time was denoted as t = 0 h when the first hyphal tips appeared in the microfluidic observation chamber. The bioreporter cells were loaded to the hyphae at t = 18 h of the noted time. (b) Corresponding pH distribution on hyphal surfaces of C. cinerea at t = 18–25 h.