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Sequence of primers and probes used for copy number determination of genetically modified soybean.
| Target sequence | Primer | Primer sequence (5′-3′) | Length |
|---|---|---|---|
| Lectin | Lectin-F | GCCCTCTACTCCACCCCCA | 118 bp |
| Lectin-R | GCCCATCTGCAAGCCTTTTT | ||
| Lectin-P | FAM AGCTTCGCCGCTTCCTTCAACTTCAC BHQ1 | ||
| GTS40-3-2 | 40-3-2-Q-1F | TTCATTCAAAATAAGATCATACATACAGGTT | 84 bp |
| 40-3-2-Q-2R | GGCATTTGTAGGAGCCACCTT | ||
| 40-3-2-Q-1P | FAM-CCTTTTCCATTTGGG-BHQ |
GTS 40-3-2 transformant-specific sequence GenBank number: HW479040.
