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. 2022 Jun 20;23(12):6867. doi: 10.3390/ijms23126867

Figure 1.

Figure 1

Effect of purified adult AD IgG on infant non-atopic intra-thymic T cells. Thymocytes from children under seven days old (n = 12) were evaluated after six days in culture in RPMI medium supplemented with FCS in the absence (mock) or presence of 100 µg/mL of commercially used purified IgG (IVIg), IgG purified from non-atopic individuals (nAT), IgG purified from atopic individuals (AT) or IgG purified from adult AD patients (AD). The frequencies of DP T, CD4 T, and CD8 T cells were evaluated (a), and the expression of CLA (b) or intracellular IFN-γ, IL-4, and IL-22 (c) were evaluated in these populations by flow cytometry. Each dot represents the value obtained from a different thymus. Bold lines represent the mean ± standard error. * p ≤ 0.05 compared to all other conditions; ** p ≤ 0.05 when compared to Mock. IVIg and nAT conditions. The absence of markings indicates that there was no statistical difference between the evaluated groups (p > 0.05).