Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Chemical compound, drug | Ang II | MCE | Cat# HY-13948 | |
| Chemical compound, drug | PE | MACKLIN | Cat# I822933 | |
| Chemical compound, drug | GSK790A | Sigma-Aldrich | Cat# G0798 | |
| Chemical compound, drug | GSK3874 | Sigma-Aldrich | Cat# SML0942 | |
| Chemical compound, drug | A21387 | Sigma-Aldrich | Cat# G0798 | |
| Chemical compound, drug | KN92 | Selleck | Cat# S6507 | |
| Chemical compound, drug | KN93 | Selleck | Cat# S6787 | |
| Chemical compound, drug | Pentobarbital sodium | Sigma-Aldrich | Cat# 76-74-4 | |
| Chemical compound, drug | Collagenase II | Worthington | Cat# ls004176 | |
| Chemical compound, drug | Fluo-4/AM | AAT Bioquest | Cat# AAT-B20401 | |
| Chemical compound, drug | Pluronic F‐127 | Solarbio | Cat# P679 | |
| Sequence-based reagent | BNP (Nppb)_R (mice) |
This paper | PCR primers | CAACTTCAGTGCGTTACAGC |
| Sequence-based reagent | Collagenase-1 (Col1a1) _F (mice) | This paper | PCR primers | GAAACCCGAGGTATGCTTGA |
| Sequence-based reagent | Collagenase1 (Col1a1) _R (mice) | This paper | PCR primers | GGGTCCCTCGACTCCTACAT |
| Sequence-based reagent | Collagenase-3 (Col3a1) _F (mice) | This paper | PCR primers | AGCCACCTTGGTCAGTCCTA |
| Sequence-based reagent | Collagenase-3 (Col3a1) _R (mice) | This paper | PCR primers | GTGTAGAAGGCTGTGGGCAT |
| Sequence-based reagent | Galectin-3 (LGALS3)_F (mice) | This paper | PCR primers | CAGGAAAATGGCAGACAGCTT |
| Sequence-based reagent | Galectin-3 (LGALS3)_R(mice) | This paper | PCR primers | CCCATGCACCCGGATATC |
| Sequence-based reagent | IL-1β (Il1b) _F (mice) | This paper | PCR primers | TGCCACCTTTTGACAGTGATG |
| Sequence-based reagent | IL-1β(Il1b)_R (mice) | This paper | PCR primers | TGATGTGCTGCTGCGAGATT |
| Sequence-based reagent | IL-6(Il6)_F (mice) | This paper | PCR primers | GATAAGCTGGAGTCACAGAAG G |
| Sequence-based reagent | IL-6(Il6)_R (mice) | This paper | PCR primers | TTGCCGAGTAGATCTCAAAGT |
| Sequence-based reagent | TNF-α(Tnfa)_F (mice) | This paper | PCR primers | CCCCAAAGGGATGAGAAGTT |
| Sequence-based reagent | TNF-α(Tnfa)_R (mice) | This paper | PCR primers | ACTTGGTGGTTTGCTACGA |
| Sequence-based reagent | MIP-2(Mip2)_F (mice) | This paper | PCR primers | CGCCCAGACAGAAGTCATAG |
| Sequence-based reagent | MIP-2(Mip2)_R (mice) | This paper | PCR primers | TCCTCCTTTCCAGGTCAGTTA |
| Sequence-based reagent | MCP-1(Mcp1p)_F (mice) | This paper | PCR primers | TTTTTGTCACCAAGCTCAAGAG |
| Sequence-based reagent | MCP-1(Mcp1p)_R (mice) | This paper | PCR primers | TTCTGATCCTCATTTGGTTCCGA |
| Sequence-based reagent | Gapdh_F(mice) | This paper | PCR primers | AAGAAGGTGGTGAAGCAGGCAT |
| Sequence-based reagent | Gapdh_F (mice) | This paper | PCR primers | CGGCATCGAAGGTGGAAGAGTG |
| Sequence-based reagent | Trpv4_F (rat) | This paper | PCR primers | CGTCCAAACCTGCGAATGAAGTTC |
| Sequence-based reagent | Trpv4_F (rat) | This paper | PCR primers | CCTCCATCTCTTGTTGTCACTGG |
| Sequence-based reagent | ANP (Nppa)_F (rat) | This paper | PCR primers | ATCTGATGGATTTCAAGAACC |
| Sequence-based reagent | ANP (Nppa)_R (rat) |
This paper | PCR primers | CTCTGAGACGGGTTGACTTC |
| Sequence-based reagent | BNP (Nppb)_F (rat) | This paper | PCR primers | CAATCCACGATGCAGAAGCT |
| Sequence-based reagent | BNP (Nppb)_R (rat) | This paper | PCR primers | GGGCCTTGGTCCTTTGAGA |
| Sequence-based reagent | Gapdh_ F (rat) | This paper | PCR primers | ATGGGAAGCTGGTCATCAAC |
| Sequence-based reagent | Gapdh_ R (rat) | This paper | PCR primers | GTGGTTCACACCCATCACAA |
| Antibody | Anti-GAPDH HRP (mouse monoclonal) |
Bioworlde | Cat# MB001H RRID: AB_2857326 |
Western blot (1:10,000) |
| Antibody | Anti-TRPV4 (rabbit polyclonal) |
Alomone labs | Cat# ACC-034 RRID: AB_2040264 |
Western blot (1:500) |
| Antibody | Anti-p-CaMKII(Thr287) (rabbit polyclonal) |
Thermo Fisher Scientific | Cat# PA5-37833 RRID: AB_2554441 |
Western blot (1:500) |
| Antibody | Anti-CaMKII (rabbit monoclonal) |
Abcam | Cat# ab52476 RRID: AB_868641 |
Western blot (1:1000) |
| Antibody | Anti-p-P65 (Ser536) (rabbit polyclonal) |
Affinity | Cat# AF2006 RRID: AB_2834435 |
Western blot (1:500) |
| Antibody | Anti-P65 (rabbit polyclonal) |
Affinity | Cat# AF5006 RRID: AB_2834847 |
Western blot (1:500) |
| Antibody | Anti-NLRP3 (rabbit monoclonal) |
Abcam | Cat# ab263899 RRID: AB_2889890 |
Western blot (1:500) |
| Antibody | Anti-ASC/TMS1 (rabbit monoclonal) |
CST | Cat# 67824 RRID: AB_2799736 |
Western blot (1:1000) |
| Antibody | Anti-Cleaved-Caspase 1, p20 (rabbit polyclonal) |
Affinity | Cat# AF4005 RRID: AB_2845463 |
Western blot (1:500) |
| Antibody | Anti-IL 1β (rabbit monoclonal) |
Abcam | Cat# ab234437 | Western blot (1:1000) |
| Antibody | Anti-IL-6 (rabbit monoclonal) |
CST | Cat# 12912 RRID: AB_2798059 |
Western blot (1:500) |
| Antibody | Anti-TNF-α (rabbit monoclonal) |
CST | Cat# 11948 RRID: AB_2687962 |
Western blot (1:1000) |
| Antibody | Goat Anti-Rabbit IgG HRP (goat polyclonal) |
Affinity | Cat# S0001 RRID: AB_2839429 |
Western blot (1:3000) |
| Antibody | Anti-α-actinin (rabbit polyclonal) |
Abcam | Cat# Ab137346 RRID: AB_2909405 |
ICC (1:500) |
| Commercial assay or kit | cDNA reverse transcription kit | Vazyme | Cat# R211-01 | |
| Commercial assay or kit | SYBR Green PCR Master Mix Kit | CWbio | Cat# cw3008h | |
| Others | Percoll | Cytiva | Cat# 17089109 | A low-viscosity, non-toxic medium suitable for density gradient centrifugation of cells, viruses and subcellular particles |