| Genetic reagent (human) |
DUB CRISPR-Cas9 screening library: Dharmacon EDIT-R crRNA Library - Human Deubiquitinating Enzymes |
Dharmacon (Horizon Discovery) |
GC-004700 Lot 17,107 |
|
| Genetic reagent (human) |
Dharmacon Edit-R tracrRNA |
Dharmacon (Horizon Discovery) |
U-002005–05 |
|
| Transfected construct |
Dharmafect 4 |
Dharmacon (Horizon Discovery) |
T-2004–02 |
|
| Cell line (human) |
MDAMB231 |
ATCC |
CRM-HTB-26 |
|
| Cell line (human) |
MCF7 |
ATCC |
HTB-22 |
|
| Antibody |
Flag-tag (L5) antibody (rat monoclonal) |
Thermo Fischer |
MA1-142 |
1:1,000 dilution |
| Antibody |
USP7 antibody (rabbit monoclonal) |
Cell Signaling |
4,833 |
1:1,000 dilution |
| Antibody |
USP8 antibody (mouse monoclonal) |
Santa Cruz Biotechnology |
sc-376130 |
1:1,000 dilution |
| Antibody |
USP10 antibody (rabbit monoclonal) |
Cell Signaling |
8,501 |
1:1,000 dilution |
| Antibody |
USP1 antibody (rabbit monoclonal) |
Cell Signaling |
D37B4 |
1:1,000 dilution |
| Antibody |
USP11 antibody (rabbit monoclonal) |
abcam |
ab109232 |
1:1,000 dilution |
| Antibody |
UCHL5 antibody (mouse monoclonal) |
Santa Cruz Biotechnology |
sc-271002 |
1:1,000 dilution |
