Table 1.
Effects of antimycin A and the fungicide Nativo® 75 WG on germination of conidia and morphology of germ tubes and appressoria of Magnaporthe oryzae Triticum at 10 μg/mL in vitro.
Treatment | Time (h) | Germination of Conidia, Morphology of Germ Tubes, and Appressorial Formation | |
---|---|---|---|
Germinated Conidia (% ± SE a) | Morphological Change/Developmental Transitions in the Treated Conidia | ||
Water | 0 | 0.0 ± 0.00 b | No germination |
6 | 100.0 ± 0.00 a | Germination with normal germ tube and normal appressoria | |
12 | 100.0 ± 0.00 a | Normal mycelial growth | |
24 | 100.0 ± 0.00 a | Normal mycelial growth | |
Antimycin A | 0 | 0.0 ± 0.00 c | No germination |
6 | 42.1 ± 0.35 a | 26.7 ± 0.41% short germ tube and 15.4 ± 0.44% conidia lysed | |
12 | 26.7 ± 0.41 b | 12.6 ± 0.40% normal germ tube, 9.3 ± 0.68% short and 4.8 ± 0.29% Abnormally elongated germ tube | |
24 | 0.0 ± 0.00 c | No appressoria, no mycelial growth | |
Nativo® 75 WG | 0 | 0.0 ± 0.00 b | No germination |
6 | 44.3 ± 2.33 a | Germinated with a short germ tube | |
12 | 44.3 ± 2.33 a | Normal germ tube | |
24 | 0.0 ± 0.00 b | No appressoria; no mycelial growth |
a The data presented here are the mean value ± SE of three replicates in each compound. Means within the column followed by the same letter(s) are not significantly different from those assessed by Tukey’s honest significance difference (HSD) post hoc (p ≤ 0.05). Conidia germination percent at different incubation times is not cumulative, but rather at different time intervals in separate experimental units.