TABLE 2.
Oligonucleotide probes used in this study
| Probe | Probe sequencea | Positionsb |
|---|---|---|
| PHORM | 5′CCTCTGGTACCGTCAGGTTGCTTTCACAA3′ | 491–472 |
| MICR#1 | 5′CCCTGAGTGTCAGATACAGCCCAGTAG3′ | 761–735 |
| MICR#2 | 5′GCAGGTGGTCAGCCAAGTCTGC3′ | 580–601 |
| MICR#3 | 5′TCTGCCAGTTTCCACCGCCTTTAGGT3′ | 649–620 |
| PLAN#2 | 5′TACAGGCCACACCTAGTTTCCATCGTTTAC3′ | 840–811 |
| ANAB | 5′CTGCTGTTAAAGAGTCTGGCTCAACCAGAT3′ | 594–625 |
| APHA | 5′CCCCTAGCTTTCGTCCCTCAGTGTCAGT3′ | 775–748 |
| NOSTOC | 5′GCTCAACCARATMARAGCAGTGGAAACTA3′ | 616–644 |
| PLAN#1 | 5′CAATCATTCCGGATAACGCTTGCATCC3′ | 565–539 |
| UNIVER | 5′CCGTMTTACCGCGGCTGCTGGCA3′ | 537–515 |
a
Primers complementary to these probe sequences were spotted on the membranes.
b
Positions are relative to the published E. coli 16S rDNA sequence (3).