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. 2022 Jun 8;12(6):859. doi: 10.3390/life12060859

Figure 3.

Figure 3

Expression of igf1ra RNA and igf1rb RNA. (A,B) RT-PCR analysis of medaka igf1ra and igf1rb in adult tissues (A) and developing embryos (B). (C–F) Ovarian and testicular cryosections using antisense igf1ra and igf1rb probes and the signals were visualized by chromogenic staining. (G,H) qPCR results of igf1ra and igf1rb at different stages of follicles. I–V, stages of oocytes; sg, spermatogonia; sc, spermatocytes; st, spermatids; sm, sperm; gc, granulosa cells; tc, theca cells; se, Sertoli cells; le, Leydig cells. Scale bars, 100 µm.