Table 3.
Summary for Forced Swim Test Immobility Time (Seconds) with URB597 Pretreatment
| Sex | Age | Control |
THC |
CBD |
THC+CBD |
F value (two-way ANOVA) |
p
|
||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Veh | Fluo | Veh | Fluo | Veh | Fluo | Veh | Fluo | ||||
| Males | P100–120 | 202.3±9.03 (n=4) | 123.8±19.87 (n=5)* | 164.5±7.43 (n=6) | 115.6±17.48 (n=8) | 164.6±8.67 (n=10) | 104.9±11.06 (n=12)** | 168.4±15.83 (n=5) | 126.1±10.30 (n=7) | F (1, 49)=33.84 | <0.0001 |
| Females | P100–120 | 182.8±13.62 (n=4) | 96.60±30.16 (n=5)* | 158.1±9.32 (n=9) | 68.60±10.20 (n=10)**** | 153.4±17.59 (n=7) | 87.91±10.16 (n=11)* | 161.8±8.80 (n=6) | 102.6±11.83 (n=8) | F (1, 52)=56.07 | <0.0001 |
| Both sexes | P100–120 | 192.5±8.41 (n=8) | 110.2±17.62 (n=10)*** | 160.7±6.19 (n=15) | 89.50±10.89 (n=18)**** | 160.0±8.63 (n=17) | 96.78±7.59 (n=23)**** | 164.8±8.229 (n=11) | 113.6±8.27 (n=15)* | F (1, 109)=87.36 | <0.0001 |
Control or PCE adult progenies were treated with vehicle or fluoxetine 30 min before FST. The data residuals were normally distributed and thus ANOVA was used for statistical analysis. Three-way ANOVA analysis found PCE and treatment but not gender as significant variables. Thus, two-way ANOVA analysis with two factors: PCE and treatment (vehicle, fluoxetine) was used. There was no interaction between these two factors. The F factor refers to the fluoxetine treatment. Post hoc Bonferroni's multiple comparisons test was conducted to compare the vehicle and fluoxetine-treated groups.
p<0.05, **p=0.01, ***p<0.001, ****p<0.0001.
FST, forced swim test; PCE, perinatal cannabinoid exposure.