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The infection of CSFV promotes the secretion of LC3B-positive vesicles. Immunofluorescence detection of autophagy proteins in EVs by confocal microscopy. PK-15 cells were infected with CSFV for 24 h, and LC3B protein which is a marker for autophagy were detected by a specific antibody (anti-LC3B, in red). Extracellular vesicles with LC3B staining signal were detected after CSFV infection. Cell nuclei were stained with DAPI (in blue).
