FIG. 1.
PCR analysis of tcpA and ctxA of genomic DNA from representative environmental strains of V. cholerae isolated from enriched APW (pH 8.5) samples. Lane 1, environmental strain of V. cholerae (SCE5) possessing tcpA-C; lane 2, environmental strain of V. cholerae (SCE340) possessing tcpA-E; lane 3, environmental strain of V. cholerae (SCE188) possessing ctxA; lane 4, V. cholerae O1 Ogawa, El Tor (positive control for ctxA and tcpA-E); lane 5, V. cholerae O1, Ogawa, classical (positive control for ctxA and tcpA-C).