TABLE 2.
Detection of CT produced by environmental strains of V. cholerae in YEP and AKI media by GM1 ELISAa
Strain or component tested | OD492
|
|
---|---|---|
YEP | AKI | |
SCE188 | 0.76 ± 0.27 | 0.35 ± 0.03 |
SCE188a | 1.78 ± 0.56 | 0.77 ± 0.20 |
SCE200 | 0.69 ± 0.24 | 0.69 ± 0.13 |
SCE201 | 0.89 ± 0.09 | 0.64 ± 0.11 |
SCE223 | 0.17 ± 0.07 | 0.18 ± 0.10 |
SCE354 | 0.06 ± 0.01 | 0.06 ± 0.01 |
VC20 | 2.43 ± 0.12 | 1.81 ± 0.42 |
SCE18 | 0.11 ± 0.04 | 0.03 ± 0.02 |
Bufferb | 0.13 ± 0.04 | 0.01 ± 0.12 |
Medium | 0.17 ± 0.02 | 0.22 ± 0.03 |
CT (1 ng) | 0.64 ± 0.03 | 0.59 ± 0.08 |
CT (0.1 ng) | 0.33 ± 0.01 | 0.25 ± 0.02 |
VC20 was a positive control, SCE18 was a negative control, and SCE188a was animal passaged.
Components (buffer, medium, CT) were tested without strains.