Figure 3.

Impaired energy metabolism in HFD-fed MBKO mice. Indirect calorimetry values of (A) oxygen consumption, (B) CO₂ production, (C) energy expenditure (EE), (D) physical activity, (E) total food intake and respiratory exchange ratio (F) (RER) of female Fl/Fl and MBKO mice fed with HFD for 14 weeks. The gray area represents nighttime (7 pm to 6 am). The average value of the parameter measured in each period was presented (*: P < 0.05, **: P < 0.01, ***: P < 0.001, two-way ANOVA, n = 6). The area under curve (AUC) of each graph was shown in the right panel of the sub-figure (*: P < 0.05, **: P < 0.01, Student’s t-test, n = 6).