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Chemical characterization of self-crosslinked oxidized alginate-carboxymethyl chitosan hydrogel with the weight ratio of 4:1 using Fourier Transform Infrared-Attenuated Total Reflectance (FTIR-ATR) showed the imine bond formation as a result of Schiff base reactions. The appearance of a new peak at 1621 cm−1 in the spectrum of hydrogel is attributed to the C=N bonds that are absent in the spectra of oxidized alginate (OAlg) and carboxymethyl chitosan (OCMC).
